CXCR2-Blocking Has Context-Sensitive Effects on Rat Glioblastoma Cell Line Outgrowth (S635) in an Organotypic Rat Brain Slice Culture Depending on Microglia-Depletion (PLX5622) and Dexamethasone Treatment

URN to cite this document:

urn:nbn:de:bvb:355-epub-551630 Copy

DOI to cite this document:

10.5283/epub.55163 Copy

Falter, Johannes ; Lohmeier, Annette ; Eberl, Petra ; Stoerr, Eva-Maria ; Koskimäki, Janne ; Falter, Lena ; Rossmann, Jakob ; Mederer, Tobias ; Schmidt, Nils Ole ; Proescholdt, Martin A.

License: Creative Commons Attribution 4.0 PDF - Published Version (4MB)

Date of publication of this fulltext: 05 Dec 2023 10:33

---

Alternative links to fulltext:DOIPublisher

---

---

Details

Item type:	Article
Open Access Type:	Gold (with APC)
Journal or Publication Title:	International Journal of Molecular Sciences
Publisher:	MDPI
Place of Publication:	BASEL
Volume:	24
Number of Issue or Book Chapter:	23
Page Range:	p. 16803
Date:	27 November 2023
Institutions:	Medicine > Lehrstuhl für Neurochirurgie
Projects (Historical):	Else-Kröner-Fresenius Stiftung (project number 3752914)
Identification Number:	Value Type 10.3390/ijms242316803 DOI
Keywords:	GLIOMA; glioblastoma; OBSC; dexamethasone; PLX5622; CXCL2; danirixin; navarixin; microglia
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	55163

Preview

Indexed in

Web of Science

Export bibliographical data

ASCII CitationAtomBibTeXData Cite XMLDataCite XML OAIDublin CoreDublin Core (LZV)EndNoteHTML CitationJSONJSON LDMETSMODSOPENAIREOpenAPCRDF+N-TriplesRDF+N3RDF+XMLReferReference ManagerSimple MetadataXMLopenCostxMetaDissPlus

---

Abstract

In glioblastoma (GBM), the interplay of different immune cell subtypes, cytokines, and/or drugs shows high context-dependencies. Interrelations between the routinely applied dexamethasone (Dex) and microglia remain elusive. Here, we exploited rat organotypic brain slice co-cultures (OBSC) to examine the effects on a rat GBM cell line (S635) outgrowth resulting from the presence of Dex and ...

Abstract

In glioblastoma (GBM), the interplay of different immune cell subtypes, cytokines, and/or drugs shows high context-dependencies. Interrelations between the routinely applied dexamethasone (Dex) and microglia remain elusive. Here, we exploited rat organotypic brain slice co-cultures (OBSC) to examine the effects on a rat GBM cell line (S635) outgrowth resulting from the presence of Dex and pretreatment with the colony-stimulating factor receptor 1 (CSF1-R) inhibitor PLX5622: in native OBSC (without PLX5622-pretreatment), a diminished S635 spheroid outgrowth was observable, whereas Dex-treatment enhanced outgrowth in this condition compared to PLX5622-pretreated OBSC. Screening the supernatants of our model with a proteome profiler, we found that CXCL2 was differentially secreted in a Dex- and PLX5622-dependent fashion. To analyze causal interrelations, we interrupted the CXCL2/CXCR2-axis: in the native OBSC condition, CXCR2-blocking resulted in increased outgrowth, in combination with Dex, we found potentiated outgrowth. No effect was found in the PLX5622-pretreated. Our method allowed us to study the influence of three different factors-dexamethasone, PLX5622, and CXCL2-in a well-controlled, simplified, and straight-forward mechanistic manner, and at the same time in a more realistic ex vivo scenario compared to in vitro studies. In our model, we showed a GBM outgrowth enhancing synergism between CXCR2-blocking and Dex-treatment in the native condition, which was levelled by PLX5622-pretreatment.

---

---

Metadata last modified: 20 Jan 2025 14:45

Owner only: item control page

Download Statistics

Download Statistics

Download Statistics

Download Statistics

Downloads

Downloads per month over past year

Altmetric

Altmetric

Altmetric

Alternative Statistics (altmetrics)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)