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The phot LOV2 domain and its interaction with LOV1

Guo, Huimin ; Kottke, Tilman ; Hegemann, Peter ; Dick, Bernhard


Phot proteins are homologs of the blue-light receptor phototropin. We report a comparative study of the photocycles of the isolated, light-sensitive domains LOV1 and LOV2 from Chlamydomonas reinhardtii phot protein, as well as the construct LOV1/2 contg. both domains. Transient absorption measurements revealed a short lifetime of the LOV2-wt triplet state (500 ns), but a long lifetime (287 ms) of ...


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