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Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 Domain

Kottke, Tilman ; Dick, Bernhard ; Fedorov, Roman ; Schlichting, Ilme ; Deutzmann, Rainer ; Hegemann, Peter



Abstract

Phot photoreceptors make up an important protein family regulating biol. processes in response to blue light. They contain two light, oxygen, and voltage sensitive (LOV) domains and a serine/threonine kinase domain. Both LOV domains noncovalently bind a FMN. Upon absorption of blue light, the LOV domains undergo a photocycle, transiently forming a covalent adduct of a cysteine residue and the FMN ...

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