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Photocycle dynamics of the Archaerhodopsin 3 based fluorescent voltage sensor Archon2

Penzkofer, Alfons ; Silapetere, Arita ; Hegemann, Peter


The retinal photocycle dynamics of the fluorescent voltage sensor Archon2 in pH 8 Tris buffer was studied. Archon2 is a mutant of Archaerhodopsin 3 (Arch) from Halorubrum sodomense obtained by a robotic multidimensional directed evolution approach (Archon2 = Arch T56P-P60S-T80P-D95H-T99S-T116I-F161V-T183IL197I-A225C). The samples were photo-excited to the first absorption band of the protonated ...


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