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Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood

Pastucha, Matej ; Odstrcilikova, Eliska ; Hlavacek, Antonin ; Brandmeier, Julian C. ; Vykoukal, Vit ; Weisova, Julie ; Gorris, Hans H. ; Skladal, Petr ; Farka, Zdenek


American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most destructive disease of the honeybee brood. Therefore, rapid and sensitive detection methods are required to limit spreading of this pathogen, which has a major impact on agriculture and biodiversity. While P. larvae is typically detected by microbial cultivation or polymerase chain reaction, antibody-based detection ...


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