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Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles

Pořízka, Pavel ; Vytisková, Karolína ; Obořilová, Radka ; Pastucha, Matěj ; Gábriš, Ivo ; Brandmeier, Julian C. ; Modlitbová, Pavlína ; Gorris, Hans H. ; Novotný, Karel ; Skládal, Petr ; Kaiser, Jozef ; Farka, Zdeněk


Immunohistochemistry (IHC) and immunocytochemistry (ICC) are widely used to identify cancerous cells within tissues and cell cultures. Even though the optical microscopy evaluation is considered the gold standard, the limited range of useful labels and narrow multiplexing capabilities create an imminent need for alternative readout techniques. Laser-induced breakdown spectroscopy (LIBS) enables ...


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