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Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Hof, Lotta ; Moreth, Till ; Koch, Michael ; Liebisch, Tim ; Kurtz, Marina ; Tarnick, Julia ; Lissek, Susanna M. ; Verstegen, Monique M. A. ; van der Laan, Luc J. W. ; Huch, Meritxell ; Matthäus, Franziska ; Stelzer, Ernst H. K. ; Pampaloni, Francesco


Background: Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective cell behaviour in mammalian organs during development, homeostasis, regeneration, and pathogenesis. To investigate the underlying cell organisation principles of organoids, we imaged hundreds of pancreas and cholangiocarcinoma ...


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