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Non-invasive and high-throughput interrogation of exon-specific isoform expression

Truong, Dong-Jiunn Jeffery ; Phlairaharn, Teeradon ; Eßwein, Bianca ; Gruber, Christoph ; Tümen, Deniz ; Baligács, Enikő ; Armbrust, Niklas ; Vaccaro, Francesco Leandro ; Lederer, Eva-Maria ; Beck, Eva Magdalena ; Geilenkeuser, Julian ; Göppert, Simone ; Krumwiede, Luisa ; Grätz, Christian ; Raffl, Gerald ; Schwarz, Dominic ; Zirngibl, Martin ; Živanić, Milica ; Beyer, Maren ; Körner, Johann Dietmar ; Santl, Tobias ; Evsyukov, Valentin ; Strauß, Tabea ; Schwarz, Sigrid C. ; Höglinger, Günter U. ; Heutink, Peter ; Doll, Sebastian ; Conrad, Marcus ; Giesert, Florian ; Wurst, Wolfgang ; Westmeyer, Gil Gregor



Abstract

Truong et al. developed a cell-based reporter system, EXSISERS, that enables non-invasive quantification of the protein expression levels of exon-specific isoforms via intein-mediated protein splicing. Expression of exon-specific isoforms from alternatively spliced mRNA is a fundamental mechanism that substantially expands the proteome of a cell. However, conventional methods to assess ...

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