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Orientational Ambiguity in Septin Coiled Coils and its Structural Basis

Leonardo, Diego A. ; Cavini, Italo A. ; Sala, Fernanda A. ; Mendonça, Deborah C. ; Rosa, Higor V.D. ; Kumagai, Patricia S. ; Crusca Jr, Edson ; Valadares, Napoleão F. ; Marques, Ivo A. ; Brandão-Neto, José ; Munte, Claudia E. ; Kalbitzer, Hans R. ; Soler, Nicolas ; Usón, Isabel ; André, Ingemar ; Araujo, Ana P.U. ; D'Muniz Pereira, Humberto ; Garratt, Richard C.


Septins are an example of subtle molecular recognition whereby different paralogues must correctly assemble into functional filaments important for essential cellular events such as cytokinesis. Most possess C-terminal domains capable of forming coiled coils which are believed to be involved in filament formation and bundling. Here, we report an integrated structural approach which aims to ...


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