; Soler Artigas, María ; Renner, Kathrin ; Hohlfeld, Jens M. ; Welte, Tobias ; Fuehner, Thomas ; Jerrentrup, Lukas ; Koczulla, Andreas Rembert ; Greulich, Timm ; Prasse, Antje ; Müller-Quernheim, Joachim ; Gupta, Sumit ; Brightling, Christopher ; Subramanian, Deepak R. ; Parr, David G. ; Kolsum, Umme ; Gupta, Vandana ; Barta, Imre ; Döme, Balázs ; Strausz, János ; Stendardo, Mariarita ; Piattella, Marco ; Boschetto, Piera ; Korzybski, Damian ; Gorecka, Dorota ; Nowinski, Adam ; Dabad, Marc
; Fernández-Callejo, Marcos ; Endesfelder, David ; zu Castell, Wolfgang ; Hiemstra, Pieter S. ; Venge, Per ; Noessner, Elfriede ; Griebel, Thasso ; Heath, Simon ; Singh, Dave ; Gut, Ivo ; Ziegler-Heitbrock, Loems | Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Scientific Reports | ||||
| Publisher: | Nature | ||||
| Place of Publication: | BERLIN | ||||
| Volume: | 11 | ||||
| Number of Issue or Book Chapter: | 1 | ||||
| Date: | 2021 | ||||
| Institutions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) | ||||
| Identification Number: |
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| Keywords: | OBSTRUCTIVE PULMONARY-DISEASE; OXIDATIVE STRESS; GRO-ALPHA; EMPHYSEMA; EPITHELIUM | ||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 56740 |
Abstract
Chronic obstructive pulmonary disease (COPD) is a destructive inflammatory disease and the genes expressed within the lung are crucial to its pathophysiology. We have determined the RNAseq transcriptome of bronchial brush cells from 312 stringently defined ex-smoker patients. Compared to healthy controls there were for males 40 differentially expressed genes (DEGs) and 73 DEGs for females with ...

Abstract
Chronic obstructive pulmonary disease (COPD) is a destructive inflammatory disease and the genes expressed within the lung are crucial to its pathophysiology. We have determined the RNAseq transcriptome of bronchial brush cells from 312 stringently defined ex-smoker patients. Compared to healthy controls there were for males 40 differentially expressed genes (DEGs) and 73 DEGs for females with only 26 genes shared. The gene ontology (GO) term "response to bacterium" was shared, with several different DEGs contributing in males and females. Strongly upregulated genes TCN1 and CYP1B1 were unique to males and females, respectively. For male emphysema (E)-dominant and airway disease (A)-dominant COPD (defined by computed tomography) the term "response to stress" was found for both sub-phenotypes, but this included distinct up-regulated genes for the E-sub-phenotype (neutrophil-related CSF3R, CXCL1, MNDA) and for the A-sub-phenotype (macrophage-related KLF4, F3, CD36). In E-dominant disease, a cluster of mitochondria-encoded (MT) genes forms a signature, able to identify patients with emphysema features in a confirmation cohort. The MT-CO2 gene is upregulated transcriptionally in bronchial epithelial cells with the copy number essentially unchanged. Both MT-CO2 and the neutrophil chemoattractant CXCL1 are induced by reactive oxygen in bronchial epithelial cells. Of the female DEGs unique for E- and A-dominant COPD, 88% were detected in females only. In E-dominant disease we found a pronounced expression of mast cell-associated DEGs TPSB2, TPSAB1 and CPA3. The differential genes discovered in this study point towards involvement of different types of leukocytes in the E- and A-dominant COPD sub-phenotypes in males and females.
Metadata last modified: 29 Feb 2024 12:37
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