Abstract
Background: The aim of this study was investigated if estrogen deficiency during puberty affects the expression of miRNA30a and miRNA503 in maxillary and mandibular growth centers, and also evaluated if ER alpha and ER beta are correlated with miRNA30a and miRNA503 expressions. Methods: Samples from 12 female Wistar rats randomized into experimental group (OVX) and control group (SHAM). At an age ...
Abstract
Background: The aim of this study was investigated if estrogen deficiency during puberty affects the expression of miRNA30a and miRNA503 in maxillary and mandibular growth centers, and also evaluated if ER alpha and ER beta are correlated with miRNA30a and miRNA503 expressions. Methods: Samples from 12 female Wistar rats randomized into experimental group (OVX) and control group (SHAM). At an age of 45 days animals were euthanized for miRNA expression analyses. RT-qPCR was performed to determine miRNA30a and miRNA503 expression in growth sites: midpalatal suture, condyle, mandibular angle, symphysis/parasymphysis and coronoid process. The data was carried out using the parametric tests at 5% of significance level. Results: miRNA 30a and miRNA503 presented higher levels in the condylar site in SHAM group when compared with OVX (p = 0.002 and p = 0.020, respectively). In the growth centers, a statistical significant difference was observed only for miRNA30a (p = 0.004), when compared mandibular angle with condyle the in OVX group (p = 0.001). A strong positive correlation between miRNA503 and ER alpha in the condyle of OVX group was observed (r = 0.90; p = 0.039 and it also between miRNA503 and ER beta in the coronoid process of the OVX group (r = 0.88; p = 0.05). Conclusion: The results suggested that estrogen regulates specific miRNAs in maxillary and mandibular growth centers, which may participate in posttranscriptional regulation of estrogen-regulated genes. (c) 2021 Published by Elsevier GmbH.
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