Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry

URN to cite this document:: urn:nbn:de:bvb:355-epub-588822
DOI to cite this document:: 10.5283/epub.58882

Weiß, Frederik ; Schnabel, Anke ; Planatscher, Hannes ; van den Berg, Bart H. J. ; Serschnitzki, Bettina ; Nuessler, Andreas K. ; Thasler, Wolfgang E. ; Weiss, Thomas S.

; Reuss, Matthias ; Stoll, Dieter ; Templin, Markus F. ; Joos, Thomas O. ; Marcus, Katrin ; Poetz, Oliver

License: Creative Commons Attribution 4.0
PDF - Published Version
(676kB)

Date of publication of this fulltext: 13 Aug 2024 12:55

Alternative links to fulltext:DOI DOI Publisher

Details

Preview

Indexed in

Export bibliographical data

Abstract

Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a ...