Gαi3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins

URN to cite this document:: urn:nbn:de:bvb:355-epub-589980
DOI to cite this document:: 10.5283/epub.58998

Weiss, Thomas S.

; Chamberlain, Chester E. ; Takeda, Tetsuro ; Lin, Ping ; Hahn, Klaus M. ; Farquhar, Marilyn Gist

License: Creative Commons Attribution Non-commercial No Derivatives 4.0
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Date of publication of this fulltext: 26 Aug 2024 09:24

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Abstract

Abstract Gαi3 is found both on the plasma membrane and on Golgi membranes. Calnuc, an EF hand protein, binds both Gαi3 and Ca2+ and is found both in the Golgi lumen and in the cytoplasm. To investigate whether Gαi3 binds calnuc in living cells and where this interaction takes place we performed fluorescence resonance energy transfer (FRET) analysis between Gαi3 and calnuc in COS-7 cells ...