| License: Creative Commons Attribution 4.0 PDF - Published Version (3MB) |
- URN to cite this document:
- urn:nbn:de:bvb:355-epub-595436
- DOI to cite this document:
- 10.5283/epub.59543
; Asam, Claudia ; Weps, Manuela ; Senninger, Antonia ; Peterhoff, David ; Bauernfeind, Stilla ; Asbach, Benedikt ; Carnell, George William ; Heeney, Jonathan Luke ; Wytopil, Monika ; Fuchs, André ; Messmann, Helmut ; Prelog, Martina ; Liese, Johannes ; Jeske, Samuel D. ; Protzer, Ulrike ; Hoelscher, Michael ; Geldmacher, Christof ; Überla, Klaus ; Steininger, Philipp ; Wagner, Ralf
; Messmann, Helmut ; Fuchs, Andre ; Ebigbo, Alanna ; Römmele, Christoph ; Ullrich, Maximilian ; Freitag, Marie ; Traidl-Hoffmann, Claudia ; Goekkaya, Mehmet ; Metz, Aline ; Holetschek, Corinna ; Neumann, Avidan ; Hoffmann, Reinhard ; Kling, Elisabeth ; Pruteanu, Mihail ; Wibmer, Thomas ; Rost, Susanne ; Überla, Klaus ; Steininger, Philipp ; Wytopil, Monika ; Beileke, Stephanie ; Müller-Schmucker, Sandra ; Korn, Klaus ; Hastreiter, Tamara ; Fraedrich, Kirsten ; Obergfäll, Debora ; Neumann, Frank ; Kuhn, Claudia ; Günther, Katja ; Friedrich, Elke ; Hoelscher, Michael ; Wieser, Andreas ; Geldmacher, Christof ; Janke, Christian ; Plank, Michael ; Guggenbühl, Jessica ; Reinkemeyer, Christina ; Noreña, Ivan ; Castelletti, Noemi ; Acero, Raquel Rubio ; Ahmed, M. I. M. ; Diepers, Paulina ; Eser, Tabea M. ; Fuchs, Anna ; Baranov, Olga ; Bauer, Bernadette ; Wang, Danni ; Paunovic, Ivana ; Protzer, Ulrike ; Jeske, Samuel D. ; Christa, Catharina ; Tinnefeld, Kathrin ; Vu, Martin ; Willmann, Annika ; Roggendorf, Hedwig ; Körber, Nina ; Bauer, Tanja ; Gleich, Sabine ; Wagner, Ralf ; Asam, Claudia ; Einhauser, Sebastian ; Weps, Manuela ; Senninger, Antonia ; Carnell, George ; Heeney, Jonathan Luke ; Ebner, Antonia ; José de Schultz, Maria ; Rajes, Cedric ; Al Wafai, Aya ; Brenner, David ; Sicheneder, Laura ; Berr, Melanie ; Schütz, Anja ; Bauernfeind, Stilla ; Hiergeist, Andreas ; Gessner, André ; Schmidt, Barbara ; Niller, Hans-Helmut ; Wenzel, Jürgen ; Biermeier, Daniela ; Lampl, Benedikt ; Rothe, Ulrich ; Gleißner, Ute ; Brückner, Susanne ; Treml, Michaela ; Schedl, Holger ; Biermaier, Beate ; Achatz, Markus ; Hierhammer, Daniela ; Englhardt, Johanna ; Scheidl, Werner ; Jeyaraman, Sivaji ; Schutt, Barbara ; Liese, Johannes ; Prelog, Martina ; Almanzar, Giovanni ; Schwägerl, Valeria ; Bley, Julia ; Vogt, Tim ; Kousha, Kimia ; Ziegler, Lars ; Stein, Astrid ; Förg, Franziska ; Löw, Johann ; Finkenberg, Barbara ; Pollak, Dennis ; Zamzow, Alexander ; Eberbach, Nicole ; Balkie, Lara ; Kretzschmann, Tanja ; Gehrig, Matthias ; Bandorf, Matthias ; Keck, Kilian ; Allmanritter, Jan ; Rafique, Shahid ; Finster, Mona ; Baumgart, Ingo ; Heumüller-Klug, Sabine ; Koglin, Hans-Jürgen ; Gefeller, Olaf ; Gall, Christine ; Pfahlberg, Annette B. ; Kaiser, Isabelle ; Scheidt, Jörg ; Drescher, Johannes ; Siebenhaar, Yannic ; Wogenstein, Florian ; Reinel, Dirk ; Weber, Beatrix ; Zarzitzky, Fabian ; Liebl, Bernhard ; Herr, Caroline ; Katz, Katharina ; Sing, Andreas ; Dangel, Alexandra | Item type: | Article | ||||
|---|---|---|---|---|---|
| Open Access Type: | DEAL (Elsevier Gold) | ||||
| Journal or Publication Title: | eBioMedicine | ||||
| Publisher: | Elsevier, The Lancet | ||||
| Volume: | 110 | ||||
| Page Range: | p. 105438 | ||||
| Date: | 9 November 2024 | ||||
| Institutions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Abteilung für Krankenhaushygiene und Infektiologie | ||||
| Identification Number: |
| ||||
| Keywords: | SARS-CoV-2, COVID-19 breakthrough infection, Neutralization, Immune imprinting, Magnitude-breadth, Antigenic map, Machine learning | ||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Partially | ||||
| Item ID: | 59543 |
Abstract
Background The impact of the infecting SARS-CoV-2 variant of concern (VOC) and the vaccination status was determined on the magnitude, breadth, and durability of the neutralizing antibody (nAb) profile in a longitudinal multicentre cohort study. Methods 173 vaccinated and 56 non-vaccinated individuals were enrolled after SARS-CoV-2 Alpha, Delta, or Omicron infection and visited four times ...

Abstract
Background
The impact of the infecting SARS-CoV-2 variant of concern (VOC) and the vaccination status was determined on the magnitude, breadth, and durability of the neutralizing antibody (nAb) profile in a longitudinal multicentre cohort study.
Methods
173 vaccinated and 56 non-vaccinated individuals were enrolled after SARS-CoV-2 Alpha, Delta, or Omicron infection and visited four times within 6 months and nAbs were measured for D614G, Alpha, Delta, BA.1, BA.2, BA.5, BQ.1.1, XBB.1.5 and JN.1.
Findings
Magnitude-breadth-analysis showed enhanced neutralization capacity in vaccinated individuals against multiple VOCs. Longitudinal analysis revealed sustained neutralization magnitude-breadth after antigenically distant Delta or Omicron breakthrough infection (BTI), with triple vaccinated individuals showing significantly elevated titres and improved breadth. Antigenic mapping and antibody landscaping revealed initial boosting of vaccine-induced WT-specific responses after BTI, a shift in neutralization towards infecting VOCs at peak responses and an immune imprinted bias towards dominating WT immunity in the long-term. Despite that bias, machine-learning models confirmed a sustained shift of the immune-profiles following BTI.
Interpretation
In summary, our longitudinal analysis revealed delayed and short lived nAb shifts towards the infecting VOC, but an immune imprinted bias towards long-term vaccine induced immunity after BTI.
Funding
This work was funded by the Bavarian State Ministry of Science and the Arts for the CoVaKo study and the ForCovid project. The funders had no influence on the study design, data analysis or data interpretation.
Metadata last modified: 04 Sep 2025 14:42
Download Statistics