Endo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sources

URN to cite this document:: urn:nbn:de:bvb:355-epub-597520
DOI to cite this document:: 10.5283/epub.59752

Hanelt, Tiana Nicole ; Treiber, Nora ; Treiber, Thomas ; Lehmann, Gerhard ; Eichner, Norbert ; Rothmeier, Tamara ; Schmid, Georg ; Reichelt, Robert ; Zambelli, Federico ; Pavesi, Giulio ; Grohmann, Dina

; Meister, Gunter

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 05 Dec 2024 06:55

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Abstract

RNA binding proteins (RBPs) are crucial regulators of gene expression and critically depend on the specific recognition of their target RNAs. Accordingly, a selection of methods to analyze RBP specificities has been developed, including protein-RNA crosslinking and sequencing (CLIP) and in vitro selection methods such as SELEX, RNA compete or RNA bind-n-seq. However, limitations like the ...