| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Dental Materials | ||||
| Publisher: | ELSEVIER SCI LTD | ||||
| Place of Publication: | OXFORD | ||||
| Volume: | 31 | ||||
| Number of Issue or Book Chapter: | 8 | ||||
| Page Range: | pp. 916-927 | ||||
| Date: | 2015 | ||||
| Institutions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie | ||||
| Identification Number: |
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| Keywords: | PULP-DERIVED CELLS; OXIDATIVE STRESS; DENTAL ADHESIVES; DIFFERENT INITIATORS; ELUTABLE SUBSTANCES; INDUCED APOPTOSIS; COMPOSITE RESINS; MONOMER ELUTION; CONVERSION; CYTOTOXICITY; Dental adhesive; Photoinitiator; TPO; CQ; Cytotoxicity; Reactive oxygen species; Apoptosis; Biocompatibility | ||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 60222 |
Web of ScienceAbstract
Objectives. The photoinitiator diphenyl-(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) is more reactive than a camphorquinone/amine (CQ) system, and TPO-based adhesives obtained a higher degree of conversion (DC) with fewer leached monomers. The hypothesis tested here is that a TPO-based adhesive is less toxic than a CQ-based adhesive. Methods. A CQ-based adhesive (SBU-CQ) (Scotchbond Universal, ...
Abstract
Objectives. The photoinitiator diphenyl-(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) is more reactive than a camphorquinone/amine (CQ) system, and TPO-based adhesives obtained a higher degree of conversion (DC) with fewer leached monomers. The hypothesis tested here is that a TPO-based adhesive is less toxic than a CQ-based adhesive. Methods. A CQ-based adhesive (SBU-CQ) (Scotchbond Universal, 3M ESPE) and its experimental counterpart with TPO (SBU-TPO) were tested for cytotoxicity in human pulp-derived cells (tHPC). Oxidative stress was analyzed by the generation of reactive oxygen species (ROS) and by the expression of antioxidant enzymes. A dentin barrier test (DBT) was used to evaluate cell viability in simulated clinical circumstances. Results. Unpolymerized SBU-TPO was significantly more toxic than SBU-CQ after a 24h exposure, and TPO alone (EC50 = 0.06 mM) was more cytotoxic than CQ (EC50 = 0.88 mM), EDMAB (EC50 = 0.68 mM) or CQ/EDMAB (EC50 = 0.50 mM). Cultures preincubated with BSO (L-buthionine sulfoximine), an inhibitor of glutathione synthesis, indicated a minor role of glutathione in cytotoxic responses toward the adhesives. Although the generation of ROS was not detected, a differential expression of enzymatic antioxidants revealed that cells exposed to unpolymerized SBU-TPO or SBU-CQ are subject to oxidative stress. Polymerized SBU-TPO was more cytotoxic than SBU-CQ under specific experimental conditions only, but no cytotoxicity was detected in a DBT with a 200 mu m dentin barrier. Significance. Not only DC and monomer-release determine the biocompatibility of adhesives, but also the cytotoxicity of the (photo-)initiator should be taken into account. Addition of TPO rendered a universal adhesive more toxic compared to CQ; however, this effect could be annulled by a thin dentin barrier. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
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