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Molecular Evolution of Broadly Neutralizing Llama Antibodies to the CD4-Binding Site of HIV-1

Desrosiers, Ronald C. ; McCoy, Laura E. ; Rutten, Lucy ; Frampton, Dan ; Anderson, Ian ; Granger, Luke ; Bashford-Rogers, Rachael ; Dekkers, Gillian ; Strokappe, Nika M. ; Seaman, Michael S. ; Koh, Willie ; Grippo, Vanina ; Kliche, Alexander ; Verrips, Theo ; Kellam, Paul ; Fassati, Ariberto ; Weiss, Robin A.



Abstract

To date, no immunization of humans or animals has elicited broadly neutralizing sera able to prevent HIV-1 transmission; however, elicitation of broad and potent heavy chain only antibodies (HCAb) has previously been reported in llamas. In this study, the anti-HIV immune responses in immunized llamas were studied via deep sequencing analysis using broadly neutralizing monoclonal HCAbs as a ...

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