Abstract
Ethnopharmacological relevance: Premna odorata Blanco (Lamiaceae) is a medicinal plant traditionally used in Albay Province, in southeastern Luzon, Philippines to treat tuberculosis. This study aimed to determine the antitubercular property of the crude extract and sub-extracts of the leaves, and to isolate the bioactive principles from the active fractions. Materials and methods: Through ...
Abstract
Ethnopharmacological relevance: Premna odorata Blanco (Lamiaceae) is a medicinal plant traditionally used in Albay Province, in southeastern Luzon, Philippines to treat tuberculosis. This study aimed to determine the antitubercular property of the crude extract and sub-extracts of the leaves, and to isolate the bioactive principles from the active fractions. Materials and methods: Through extraction, solvent polarity-based fractionation and silica gel chromatography purification of the DCM sub-extract, compound mixtures from the bioactive fractions were isolated and screened for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H(37)Rv using the colorimetric Microplate Alamar Blue assay (MABA). Results: The crude methanolic extract and sub-extracts showed poor inhibitory activity against Mycobacterium tuberculosis H(37)Rv (MIC >= 128 mu g/mL). However, increased inhibitory potency was observed for fractions eluted from the DCM sub-extract (MIC=54 to 120 mu g/mL). Further purification of the most active fraction (MIC=54 mu g/mL) led to the isolation of a 1-heneicosyl formate (1), 4:1 mixture of p-sitosterol (2), stigmasterol (3) and diosmetin (4), which were identified through GC MS analysis (with dereplication) and NMR experiments. The MIC of compound 1 was 8 mu g/mL. Conclusions: The results of this study provide scientific basis for the traditional use of Premna odorata as treatment for tuberculosis. (C) 2014 Published by Elsevier Ireland Ltd.
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