| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Journal of Cardiothoracic and Vascular Anesthesia | ||||
| Publisher: | W B SAUNDERS CO-ELSEVIER INC | ||||
| Place of Publication: | PHILADELPHIA | ||||
| Volume: | 27 | ||||
| Number of Issue or Book Chapter: | 3 | ||||
| Page Range: | pp. 494-501 | ||||
| Date: | 2013 | ||||
| Institutions: | Medicine > Lehrstuhl für Anästhesiologie | ||||
| Identification Number: |
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| Keywords: | ISCHEMIA-REPERFUSION INJURY; MYOCARDIAL INFARCT SIZE; IN-VIVO; DELAYED CARDIOPROTECTION; DEPENDENT MECHANISM; UP-REGULATION; MICE LACKING; ISOFLURANE; METAANALYSIS; INHIBITION; anesthetic-induced preconditioning; myocardial infarction; endothelial nitric oxide synthase; inducible nitric oxide synthase; mouse | ||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 62543 |
Web of ScienceAbstract
Objectives: Nitric oxide synthases (NOSs) mediate the first window of anesthetic-induced preconditioning (APC). The authors tested the hypothesis that endothelial NOS (eNOS) mediates the first window and inducible NOS (iNOS) mediates the second window of APC. Design: Randomized, prospective, blinded laboratory investigation. Setting: Experimental laboratory. Participants: Mice. Interventions: ...
Abstract
Objectives: Nitric oxide synthases (NOSs) mediate the first window of anesthetic-induced preconditioning (APC). The authors tested the hypothesis that endothelial NOS (eNOS) mediates the first window and inducible NOS (iNOS) mediates the second window of APC. Design: Randomized, prospective, blinded laboratory investigation. Setting: Experimental laboratory. Participants: Mice. Interventions: Mice were subjected to a 45-minute coronary artery occlusion (CAO) and a 180-minute reperfusion. C57BL/6 mice received desflurane, 1.0 minimum alveolar concentration, for 30 minutes or 12, 24, 48, or 96 hours before GAO. In eNOS(-/-) and iNOS(-/-) mice, desflurane was given 30 minutes and 48 hours before GAO. In the control groups, no desflurane was administered. Myocardial infarct size (IS) was determined after staining with Evans blue and triphenyltetrazolium chloride. Measurements and Main Results: The second window of APC was detectable at 48 hours but not at 12, 24, and 96 hours after preconditioning. In the control groups, IS was not different among the wild-type (50 +/- 10%), eNOS(-/-) (52 +/- 14%), and iNOS(-/-) (46 +/- 10%) mice. The IS decreased significantly (p < 0.05) when desflurane was administered 30 minutes (10 +/- 6%) or 48 hours (16 +/- 7%) before CAO in wild-type mice, 48 hours (21 +/- 13%) before GAO in eNOS(-/-) mice, and 30 minutes (13 +/- 6%) before GAO in iNOS(-/-) mice. Desflurane given 30 minutes before CAO in eNOS(-/-) mice (60 +/- 10%) and 48 hours before CAO in iNOS(-/-) mice (48 21%) did not decrease the IS significantly compared with controls. Conclusions: Endothelial NOS and iNOS work independently to mediate the first and second windows of APC, respectively. Endothelial NOS is not necessary to trigger the second window of APC. (C) 2013 Elsevier Inc. All rights reserved.
Metadata last modified: 19 Dec 2024 08:39
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