Abstract
We introduce two water-soluble ruthenium complexes, RuPD and RuPP, and show their potential as probes for luminescent recognition of diacetyl. The recognition involves the reaction of diacetyl with a diamine moiety to convert the almost nonluminescent complex into a stronger emitting species with an up to 31-fold increase of luminescence. The incubation time could be reduced by a factor of 2.5 ...
Abstract
We introduce two water-soluble ruthenium complexes, RuPD and RuPP, and show their potential as probes for luminescent recognition of diacetyl. The recognition involves the reaction of diacetyl with a diamine moiety to convert the almost nonluminescent complex into a stronger emitting species with an up to 31-fold increase of luminescence. The incubation time could be reduced by a factor of 2.5 with respect to our former rhodamine B-based probe for diacetyl. Moreover, RuPD is the first probe that permits determination of diacetyl in aqueous buffer at neutral pH and (even more sensitive) at acidic pH in micromolar concentrations. RuPD reacts more selectively with diacetyl than with other carbonyls and shows longwave emission at 625 nm. This provides an assay for diacetyl that is hardly prone to co-excited background luminescence in biological environments as shown by its application in spiked samples of cell nutrition medium. (C) 2012 Elsevier B.V. All rights reserved.
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