Abstract
Background: Mice lacking Foxp3(+) regulatory T (Treg) cells develop severe tissue inflammation in lung, skin, and liver with premature death, whereas the intestine remains uninflamed. This study aims to demonstrate the importance of Foxp3+ Treg for the activation of T cells and the development of intestinal inflammation. Methods: Foxp3-GFP-DTR (human diphtheria toxin receptor) C57BL/6 mice allow ...
Abstract
Background: Mice lacking Foxp3(+) regulatory T (Treg) cells develop severe tissue inflammation in lung, skin, and liver with premature death, whereas the intestine remains uninflamed. This study aims to demonstrate the importance of Foxp3+ Treg for the activation of T cells and the development of intestinal inflammation. Methods: Foxp3-GFP-DTR (human diphtheria toxin receptor) C57BL/6 mice allow elimination of Foxp3(+) Treg by treatment with Dx (diphtheria toxin). The influence of Foxp3(+) Treg on intestinal inflammation was tested using the CD4(+) T-cell transfer colitis model in Rag(-/-) C57BL/6 mice and the acute DSS-colitis model. Results: Continuous depletion of Foxp3(+) Treg in Foxp3-GFP-DTR mice led to dramatic weight loss and death of mice by day 28. After 10 days of depletion of Foxp3(+) Treg, isolated CD4(+) T-cells were activated and produced extensive amounts of IFN-gamma, IL-13, and IL-17A. Transfer of total CD4(+) T-cells isolated from Foxp3-GFP-DTR mice did not result in any changes of intestinal homeostasis in Rag(-/-) C57BL/6 mice. However, administration of DTx between days 14 and 18 after T-cell reconstitution, lead to elimination of Foxp3(+) Treg and to immediate weight loss due to intestinal inflammation. This pro-inflammatory effect of Foxp3(+) Treg depletion consecutively increased inflammatory cytokine production. Further, the depletion of Foxp3(+) Treg from Foxp3-GFP-DTR mice increased the severity of acute dSS-colitis accompanied by 80% lethality of Treg-depleted mice. CD4(+) effector T-cells from Foxp3(+) Treg-depleted mice produced significantly more pro-inflammatory cytokines. Conclusion: Intermittent depletion of Foxp3(+) Treg aggravates intestinal inflammatory responses demonstrating the importance of Foxp3(+) Treg for the balance at the mucosal surface of the intestine.
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