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Resazurin Dye as a Reliable Tool for Determination of Cell Number and Viability in Mesenchymal Stem Cell Culture

Dienstknecht, T. ; Ehehalt, K. ; Jenei-Lanzl, Zsuzsa ; Zellner, J. ; Müller, Michael ; Berner, A. ; Nerlich, M. ; Angele, P.



Abstract

Human mesenchymal stem cells are a valuable cell source for tissue engineering. Determination of cell number and viability is crucial. However, this can be tested only at the end of cell culture. This study shows that Resazurin dye staining is a reliable tool for evaluation of cell number and viability in culture without cell perturbation.


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