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Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

Armache, Jean-Paul ; Jarasch, Alexander ; Anger, Andreas M. ; Villa, Elizabeth ; Becker, Thomas ; Bhushan, Shashi ; Jossinet, Fabrice ; Habeck, Michael ; Dindar, Gülcin ; Franckenberg, Sibylle ; Marquez, Viter ; Mielke, Thorsten ; Thomm, Michael ; Berninghausen, Otto ; Beatrix, Birgitta ; Söding, Johannes ; Westhof, Eric ; Wilson, Daniel N. ; Beckmann, Roland



Abstract

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum ...

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