| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Annals of Biomedical Engineering | ||||
| Publisher: | SPRINGER | ||||
| Place of Publication: | NEW YORK | ||||
| Volume: | 38 | ||||
| Number of Issue or Book Chapter: | 6 | ||||
| Page Range: | pp. 2197-2209 | ||||
| Date: | 2010 | ||||
| Institutions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth | ||||
| Identification Number: |
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| Keywords: | LAEVIS CULTURED-CELLS; HEAT-SHOCK-PROTEIN; ARTIFICIAL INTERSTITIUM; MESENCHYMAL TRANSITION; URETERAL BUD; DISEASE; FIBROBLASTS; EPITHELIUM; EXPRESSION; INDUCTION; Tissue engineering; Perfusion culture; Kidney; Tubule; Artificial interstitium; Collagen type III | ||||
| Dewey Decimal Classification: | 500 Science > 570 Life sciences | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 66030 |
Web of ScienceAbstract
An increasing number of investigations is dealing with the repair of acute and chronic renal failure by the application of stem/progenitor cells. However, accurate data concerning the cell biological mechanisms controlling the process of regeneration are scarce. For that reason new implantation techniques, advanced biomaterials and morphogens supporting regeneration of renal parenchyma are under ...
Abstract
An increasing number of investigations is dealing with the repair of acute and chronic renal failure by the application of stem/progenitor cells. However, accurate data concerning the cell biological mechanisms controlling the process of regeneration are scarce. For that reason new implantation techniques, advanced biomaterials and morphogens supporting regeneration of renal parenchyma are under research. Special focus is directed to structural and functional features of the interface between generating tubules and the surrounding interstitial space. The aim of the present experiments was to investigate structural features of the interstitium during generation of tubules. Stem/progenitor cells were isolated from neonatal rabbit kidney and mounted between layers of a polyester fleece to create an artificial interstitium. Perfusion culture was performed for 13 days in chemically defined Iscove's Modified Dulbecco's Medium containing aldosterone (1 x 10(-7) M) as tubulogenic factor. Recordings of the artificial interstitium in comparison to the developing kidney were performed by morphometric analysis, scanning and transmission electron microscopy. The degree of differentiation was registered by immunohistochemistry. The data reveal that generated tubules are embedded in a complex network of fibers consisting of newly synthesized extracellular matrix proteins. Morphometric analysis further shows that the majority of tubules within the artificial interstitium develops in a surprisingly close distance between 5 and 25 mu m to each other. The abundance of synthesized extracellular matrix acts obviously as a spacer keeping generated tubules in distance. For comparison, the same principle of construction is found in the developing parenchyma of the neonatal kidney. Most astonishingly, scanning electron microscopy reveals that the composition of interstitial matrix is not homogeneous but differs along a cortico-medullary axis of proceeding tubule development.
Metadata last modified: 19 Dec 2024 11:35
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