Synthesis of DNA with Spirobenzopyran as an Internal Covalent Modification

Zusammenfassung

The photochromic spirobenzopyran was incorporated as an internal modification into oligonucleotides by two different synthetic strategies: For the first route the spiropyran phosphoramidite was synthesized as a DNA building block, whereas for the second route the postsynthetic 'click'-type ligation was applied. Photoinduced ring opening of the chromophore could not be achieved in duplex DNA.