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Preliminary validation of real-time PCR assays for the identification of Yersinia pestis

Tomaso, Herbert ; Jacob, Daniela ; Eickhoff, Meike ; Scholz, Holger C. ; Al Dahouk, Sascha ; Kattar, Mireille M. ; Reischl, Udo ; Plicka, Helga ; Olsen, Jaran Strand ; Nikkari, Simo ; Matero, Pirjo ; Beuret, Christian ; Ciammaruconi, Andrea ; Lista, Florigio ; Gala, Jean-Luc ; Broll, Hermann ; Appel, Bernd ; Sellek Cano, Ricela E. ; Ybarra de Villavicencio, Maria del Carmen ; Broekhuijsen, Martien ; Indra, Alexander ; Petersen, Roger ; Neubauer, Heinrich



Abstract

Background: Yersinia pestis (Y. pestis) is a zoonotic bacterium mainly circulating among rodents and their fleas. Transmission to humans can cause bubonic, pneumonic or septicemic plague with a high case-fatality rate. Therefore, rapid and reliable diagnostic tools are crucial. The objective of this study was to assess the inter-laboratory reproducibility of in-house developed real-time PCR ...

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