Abstract
Toxicity potentiation of two monomers [bisphenol-A-glycidyldimethacrylate (BisGMA) and urethanedimethacrylate (UDMA)] as well as two comonomers [triethyleneglycoldimethacrylate (TEGDMA) and 2-hydroxyethylmethacrylate (HEMA)], each in combination with H2O2, was investigated on the viability on human gingival fibroblasts (HGF) and human pulpal fibroblasts (HPF). The applied concentration of H2O2 ...
Abstract
Toxicity potentiation of two monomers [bisphenol-A-glycidyldimethacrylate (BisGMA) and urethanedimethacrylate (UDMA)] as well as two comonomers [triethyleneglycoldimethacrylate (TEGDMA) and 2-hydroxyethylmethacrylate (HEMA)], each in combination with H2O2, was investigated on the viability on human gingival fibroblasts (HGF) and human pulpal fibroblasts (HPF). The applied concentration of H2O2 was 0.06 or 0.1 mmol/l, respectively, corresponding to the EC0 of H2O2 in HGF or HPF. The cell viability was assessed by the XTT test. From this test the half maximum effect concentrations (EC50) were calculated from fitted sigmoidale curves. EC50 values were (HGF; mmol/l; mean +/- s.e.m.; n = 5): HEMA 11.9 +/- 0.9, TEGDMA 3.7 +/- 0.3, H2O2 0.36 +/- 0.04, UDMA 0.27 +/- 0.08, and BisGMA 0.11 +/- 0.03. No significant (P < 0.05) differences in the EC50 values were observed when HGF was exposed to substances, as compared to HPF. No significant decrease of the EC50 values was found when HGF or HPF, respectively, was exposed to HEMA or BisGMA in addition with H2O2 up to the concentration of 0.1 mmol/l, as compared to those EC50 values of each compound without H2O2 addition. A significant decrease of the TEGDMA EC50 value from 3.7 to 2.1 or 0.4 mmol/l, respectively, was found when cells were exposed to TEGDMA in combination with H2O2 (0.06 or 0.1 mmol/l), as compared to that TEGDMA EC50 value without H2O2 addition. A significant decrease of the UDMA EC50 value from 0.27 to 0.11 or 0.08 mmol/l, respectively, was found when HGF or HPF was exposed to UDMA in combination with H2O2 (0.06 or 0.1 mmol/l), as compared to that UDMA EC50 value without H2O2 addition. The addition of H2O2 (0.06 or 0.1 mmol/l) resulted in a toxicity potentiation of TEGDMA and UDMA, but not of HEMA and BisGMA, on HGF or HPF.
Altmetric