Abstract
The fluorescence behaviour of the flavins riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and lumiflavin in aqueous solution at pH 8 in the presence of the reducing agents beta-mercaptoethanol (beta-ME), dithiothreitol (DTT), and sodium nitrite (NaNO2) is studied under aerobic conditions. The fluorescence quantum yields and fluorescence lifetimes are determined as a ...
Abstract
The fluorescence behaviour of the flavins riboflavin, flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and lumiflavin in aqueous solution at pH 8 in the presence of the reducing agents beta-mercaptoethanol (beta-ME), dithiothreitol (DTT), and sodium nitrite (NaNO2) is studied under aerobic conditions. The fluorescence quantum yields and fluorescence lifetimes are determined as a function of the reducing agent concentration. For all three reducing agents diffusion controlled dynamic fluorescence quenching is observed which is thought to be due to photo-induced reductive electron transfer. For DTT additionally static fluorescence quenching occurs. (c) 2007 Elsevier B.V. All rights reserved.
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