Abstract
Background. The etiology of malignomas of human salivary glands is examined. Material and methods. Macroscopic, healthy salivary gland tissue from 46 donors was harvested during surgery. Single cells were isolated by enzymatic digestion. These were then incubated for 60 min with Na2Cr2O7, NiSO4, CdSO4, ZnCl2 and ethanol. Additionally, incubation with Na2Cr2O7 was combined with NiSO4, CdSO4, ZnCl2 ...
Abstract
Background. The etiology of malignomas of human salivary glands is examined. Material and methods. Macroscopic, healthy salivary gland tissue from 46 donors was harvested during surgery. Single cells were isolated by enzymatic digestion. These were then incubated for 60 min with Na2Cr2O7, NiSO4, CdSO4, ZnCl2 and ethanol. Additionally, incubation with Na2Cr2O7 was combined with NiSO4, CdSO4, ZnCl2 and ethanol. The influence of CdSO4 was analyzed by altered combinations with Na2Cr2O7 during incubation and by the DNA-repair period. Evaluation was performed using fluorescent staining and digital analysis. Results. Of all of the substances tested, only Na2Cr2O7 showed genotoxic effects. NiSO4, ZnCl2 and ethanol had neither genotoxic nor cofactorial impacts. CdSO4, however, caused additional genotoxic effects in combination with Na2Cr2O7, although it lacked direct genotoxic potential. A reduction of DNA-repair of Na2Cr2O7-induced oxidative damage by CdSO4 could be demonstrated. Conclusions. In this investigation, sodium dichromate was identified as genotoxic in association with human salivary gland tissue. These effects could be increased by CdSO4, reinforcing DNA damage based on oxidative stress.
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