| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Journal of Experimental Biology | ||||
| Publisher: | COMPANY OF BIOLOGISTS LTD | ||||
| Place of Publication: | CAMBRIDGE | ||||
| Volume: | 206 | ||||
| Number of Issue or Book Chapter: | 9 | ||||
| Page Range: | pp. 1575-1588 | ||||
| Date: | 2003 | ||||
| Institutions: | Biology, Preclinical Medicine > Institut für Zoologie | ||||
| Identification Number: |
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| Keywords: | OLFACTORY RECEPTOR NEURONS; LOCUST LOCUSTA-MIGRATORIA; INSECT ANTENNAE; BOMBYX-MORI; CYCLIC-GMP; G-PROTEINS; RESPONSES; COMPONENTS; LEPIDOPTERA; OCTOPAMINE; Manduca sexta; insect olfaction; adaptation; pheromone sensillum; tip recording; bombykal; action potential; sensillar potential | ||||
| Dewey Decimal Classification: | 500 Science > 590 Zoological sciences | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 72291 |
Web of ScienceAbstract
In extracellular tip recordings from long trichoid sensilla of male Manduca sexta moths, we studied dose-response relationships in response to bombykal stimuli of two different durations in the adapted and the non-adapted state. Bombykal-responsive cells could be distinguished from non-bombykal-sensitive cells in each trichoid sensillum because the bombykal-responsive cell always generated the ...
Abstract
In extracellular tip recordings from long trichoid sensilla of male Manduca sexta moths, we studied dose-response relationships in response to bombykal stimuli of two different durations in the adapted and the non-adapted state. Bombykal-responsive cells could be distinguished from non-bombykal-sensitive cells in each trichoid sensillum because the bombykal-responsive cell always generated the action potentials of larger initial amplitude. The bombykal cell, which was recorded at a defined location within a distal flagellar annulus, can resolve at least four log(10)-units of pheromone concentrations but is apparently unable to encode all stimulus durations tested. Parameters of the amplitude-modulated sensillar potential and the frequency-modulated action potential responses were examined in different states of adaptation. Evidence is presented for the existence of several mechanisms of adaptation, which affect distinct steps of the transduction cascade. After adapting pheromone stimuli, the sensillar potential rises to a lower amplitude and declines faster compared with the non-adapted response. In addition, the frequency of the adapted action potential response is reduced. Only the time of rise of the sensillar potential is differentially affected by adapting pheromone stimuli of different duration. The time of rise does not increase after short, but only after long, adapting stimuli. Both short and long adapting stimuli shift the dose-response curves of the sensillar potential amplitude, as well as the initial slope of its rising phase, to higher stimulus concentrations by approximately one log(10)-unit. The shift in the dose-response curve of the action potential response is larger than for the sensillar potential response, suggesting that an additional adaptation mechanism acts at the level of action potential generation. Furthermore, a faster decline of the sensillar potential after short and long adapting stimuli suggests that the resting potential of the olfactory receptor neuron is stabilized.
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