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Detection of protein phosphorylation on SDS-PAGE using probes with a phosphate-sensitive emmision response

Riechers, Alexander ; Schmidt, Florian ; Stadlbauer, Stefan ; König, Burkhard



Abstract

Fluorescent probes for the detection of protein phosphorylation on SDS-PAGE are presented. The probes were designed using a dinuclear metal-chelate phosphate recognition unit and an environmentally sensitive fluorophore. The specificity of the probes is determined by their binding site selectivity toward phosphate ions and the emission response induced by the change in the electrostatic ...

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