Abstract
The rotation angle/absorbance ratios C(+) = alpha (+)/A(+) and C(-) = alpha (-)/A(-), determined via,detection by a polarimeter and a photometer, were checked for the first time with reference to their use for on-line analysis during preparative separations. For this purpose, (+)-, (-)- and (+/-)-carvones were investigated by liquid chromatography (LC) on microcrystalline tribenzoylcelulose. It ...
Abstract
The rotation angle/absorbance ratios C(+) = alpha (+)/A(+) and C(-) = alpha (-)/A(-), determined via,detection by a polarimeter and a photometer, were checked for the first time with reference to their use for on-line analysis during preparative separations. For this purpose, (+)-, (-)- and (+/-)-carvones were investigated by liquid chromatography (LC) on microcrystalline tribenzoylcelulose. It turned out that the ratios C(+) and C(-) depend only slightly upon concentration (Table I). Overlapped peaks of enantiomers were successfully submitted to computer deconvolution (e.g. Pig. 2, bottom). A procedure for on-line analysis during preparative LC is proposed. (C) 2001 Elsevier Science B.V. All rights reserved.
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