Catalysis of GTP Hydrolysis by Small GTPases at Atomic Detail by Integration of X-ray Crystallography, Experimental, and Theoretical IR Spectroscopy

URN to cite this document:: urn:nbn:de:bvb:355-epub-751398
DOI to cite this document:: 10.5283/epub.75139

Rudack, Till ; Jenrich, Sarah ; Brucker, Sven ; Vetter, Ingrid R. ; Gerwert, Klaus ; Kötting, Carsten

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Date of publication of this fulltext: 20 Mar 2025 05:29

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Abstract

Small GTPases regulate key processes in cells. Malfunction of their GTPase reaction by mutations is involved in severe diseases. Here, we compare the GTPase reaction of the slower hydrolyzing GTPase Ran with Ras. By combination of time-resolved FTIR difference spectroscopy and QM/MM simulations we elucidate that the Mg2+ coordination by the phosphate groups, which varies largely among the x-ray ...