Design of an Ultrafast G Protein Switch Based on a Mouse Melanopsin Variant

URN to cite this document:: urn:nbn:de:bvb:355-epub-751486
DOI to cite this document:: 10.5283/epub.75148

Tennigkeit, Stefan Alexander

; Karapinar, Raziye ; Rudack, Till

; Dreier, Max‐Aylmer

; Althoff, Philipp ; Eickelbeck, Dennis

; Surdin, Tatjana ; Grömmke, Michelle ; Mark, Melanie D.

; Spoida, Katharina ; Lübben, Mathias

; Höweler, Udo ; Herlitze, Stefan

; Gerwert, Klaus

License: Creative Commons Attribution 4.0
PDF - Published Version
(5MB)

Date of publication of this fulltext: 20 Mar 2025 06:04

Alternative links to fulltext:DOI Publisher

Details

Preview

Export bibliographical data

Abstract

The primary goal of optogenetics is the light-controlled noninvasive and specific manipulation of various cellular processes. Herein, we present a hybrid strategy for targeted protein engineering combining computational techniques with electrophysiological and UV/visible spectroscopic experiments. We validated our concept for channelrhodopsin-2 and applied it to modify the less-well-studied ...