The aim of this article is to deepen the discussion on the nature and mechanisms of culture change based on the analysis of newly acquired materials from the Targowisko settlement region. Three groups of materials were acquired (from narrow time horizons) related to the single-phase relics of Linienbandkeramik (Brzezie, site 40 and Targowisko, site 16) and Malice culture houses (Targowisko, site 14-15). The absolute chronology of the beginning of the late phase (III) LBK was established to be 5100-5000 BC, and the classic phase (Ib) of MC was dated to 4650-4550 BC. Selected threads of the cultural tradition (in the field of ceramic-making technology and ornamentation and flint-blade production technology) were passed on among families living in individual houses. Settlement analysis showed the relative instability of microregions, the increased mobility of small groups of people, and risky colonization attempts in Targowisko region. No evidence of direct, contemporaneous contact between the LBK and MC populations was found.