Immunity to MHC class I antigen after direct DNA transfer into skeletal muscle.

URN to cite this document:: urn:nbn:de:bvb:355-epub-95547
DOI to cite this document:: 10.5283/epub.9554

Geissler, Edward K. ; Wang, Jue ; Fechner, John H. ; Burlingham, William J. ; Knechtle, Stuart J.

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Date of publication of this fulltext: 22 Sep 2009 12:10

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Abstract

Plasmid cDNA encoding the alpha-chain of either membrane-bound (pcRT.45) or secreted (pcRQ.B3) RT1Aa MHC class I Ag were transferred to Lewis (RT1(1)) rat skeletal muscle by direct injection. Rats were challenged 7 days later with an ACI (RT1a) heterotropic heart transplant, and cardiac allograft survival, RT1Aa-specific antibody levels, and frequency of ACI-specific CTL were monitored. Graft ...