Single Molecule Fluorescence Spectroscopy at Ambient Temperature: Design and Application of a Time-Resolved Confocal Microscope with Single-Molecule Sensitivity

URN to cite this document:: urn:nbn:de:bvb:355-opus-724
DOI to cite this document:: 10.5283/epub.9917

Böhmer, Martin

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Date of publication of this fulltext: 16 May 2002 13:41

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Item type:	Thesis of the University of Regensburg (PhD)
Open Access Type:	Primary Publication
Date:	15 May 2002
Referee:	Jörg Enderlein
Date of exam:	4 February 2002
Institutions:	Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik
Keywords:	Fluoreszenz , Spektroskopie , Mikroskopie , Einzelmolekülspektroskopie , Single Molecule Spectroscopy
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	9917

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Abstract (German)

Diese Dissertation beschreibt die Entwicklung und Konstruktion eines zeitaufgelösten konfokalen Laser-Scanning Mikroskops für die hoch sensitive Fluoreszenzdetektion in Lösung und auf Oberflächen. Mit diesem Mikroskop lassen sich einzelne, fluoreszierende Moleküle in Lösungen und auf Oberflächen detektieren.

Translation of the abstract (English)

This thesis describes the development and construction of a time-resolved confocal laser-scanning microscope for ultrasensive fluorescence detection in solution and on surfaces. The system is capable of monitoring single molecules in liquids and on surfaces, capable of performing emission and/or polarization spectroscopy, time-resolved spectroscopy with picosecond temporal resolution, and ...