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Single Molecule Fluorescence Spectroscopy at Ambient Temperature: Design and Application of a Time-Resolved Confocal Microscope with Single-Molecule Sensitivity

URN to cite this document:
Böhmer, Martin
Date of publication of this fulltext: 16 May 2002 13:41

Abstract (German)

Diese Dissertation beschreibt die Entwicklung und Konstruktion eines zeitaufgelösten konfokalen Laser-Scanning Mikroskops für die hoch sensitive Fluoreszenzdetektion in Lösung und auf Oberflächen. Mit diesem Mikroskop lassen sich einzelne, fluoreszierende Moleküle in Lösungen und auf Oberflächen detektieren.

Translation of the abstract (English)

This thesis describes the development and construction of a time-resolved confocal laser-scanning microscope for ultrasensive fluorescence detection in solution and on surfaces. The system is capable of monitoring single molecules in liquids and on surfaces, capable of performing emission and/or polarization spectroscopy, time-resolved spectroscopy with picosecond temporal resolution, and ...


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