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High throughput quantification of cholesterol and cholesteryl ester by electrospray ionization tandem mass spectrometry (ESI-MS/MS)

Liebisch, Gerhard and Binder, Marion and Schifferer, Rainer and Langmann, Thomas and Schulz, Berta and Schmitz, Gerd (2006) High throughput quantification of cholesterol and cholesteryl ester by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Biochimica et biophysica acta 1761 (1), pp. 121-128.

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Other URL: http://dx.doi.org/10.1016/j.bbalip.2005.12.007


Abstract

Analysis of free cholesterol (FC) is not well suited for electrospray ionization (ESI); however, cholesteryl ester (CE) form ammonium adducts in positive ion mode and generate a fragment ion of m/z 369 upon collision-induced fragmentation. In order to allow parallel analysis of FC and CE using ESI tandem mass spectrometry (ESI-MS/MS), we developed an acetyl chloride derivatization method to ...

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Item Type:Article
Date:January 2006
Institutions:Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin
Identification Number:
ValueType
16458590PubMed ID
10.1016/j.bbalip.2005.12.007DOI
Keywords:Cholesterol; Cholesteryl ester; Lipid; Stable isotope labeling; Lipidomics; Sterol; Mass spectrometry; Electrospray ionization
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Ute Lange
Deposited On:01 Mar 2007
Last Modified:13 Mar 2014 09:44
Item ID:1020
Owner Only: item control page
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