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Solid-state 31P NMR of nucleotide binding proteins

URN to cite this document: urn:nbn:de:bvb:355-opus-4432

Iuga, Adriana (2004) Solid-state 31P NMR of nucleotide binding proteins. PhD, Universität Regensburg

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Abstract (English)

The major results of the present work are related to low molecular weight guanosine triphosphate (GTP) binding Ras proteins which play an essential role in a variety of diverse cellular signal transduction and transport processes by cycling between a GTP-bound �on� state and a guanosine diphosphate (GDP) bound �off� state. Ras(wt)·Mg2+·GppNHp exists in two biological relevant conformational states: state 1 and state 2. State 1 is an "open", disordered conformation of the switch regions similar to the GDP-bound state. State 2 appears to be very similar to the conformation of Ras in the "on" state which is observed for Ras interacting with effector proteins. It was shown that the wild-type protein molecules preferentially exist in state 2. Mutant protein molecules preferentially exist in the "open" state 1.

Translation of the abstract (German)

Das Guaninnukleotid-bindende Protein Ras ist als molekularer Schalter in zellulären Signaltransduktionswegen involviert. Ras(wt) kann in der aktiven Konformation in zwei konformationellen Zuständen vorliegen (Zustand 1 und 2), die sich im chemischen Austausch miteinander befinden. Das Gleichgewicht zwischen den beiden Zuständen wird durch Mutationen in der Schalter I-Region von Ras zum Zustand 1 verschoben.

Item Type:	Thesis of the University of Regensburg (PhD)
Referee:	Eike (Prof. Dr.) Brunner
Date of exam:	07 December 2004
Institutions:	Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Eike Brunner
Keywords:	Ras-Proteine , Festkörper-NMR-Spektroskopie , Molekulardynamik , , Ras protein , conformational exchange , solid-state 31P NMR
Subjects:	500 Science > 570 Life sciences
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Owner:	Universitätsbibliothek Regensburg
Deposited On:	26 Oct 2009 16:38
Last Modified:	17 Oct 2012 11:05
Item ID:	10243

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