Orsó, Evelyn and Werner, Tobias and Wolf, Zsuzsanna and Bandulik, Sascha and Kramer, Werner and Schmitz, Gerd (2006) Ezetimib influences the expression of raft-associated antigens in human monocytes. Cytometry. Part A : the journal of the International Society for Analytical Cytology 69 (3), pp. 206-208.
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Other URL: http://dx.doi.org/10.1002/cyto.a.20229
Aminopeptidase N (CD13) was recently identified as a molecular target of the cholesterol absorption inhibitor Ezetimib. Regarding that CD13 is expressed in lipid rafts of monocytic cells, we have investigated whether Ezetimib influences raft function in these cells. Expression of raft-associated antigens (CD11b, CD13, CD14, CD16, CD36, and CD64) was followed by flow cytometry and/or immunoblot in human monocyte-derived macrophages in response to in vitro administration of Ezetimib. Cellular redistribution of CD13 was assessed by confocal imaging. Ezetimib significantly decreased the surface expression of CD13, CD16, CD64, and CD36; furthermore, it induced a shift of CD13 from plasma membrane to intracellular vesicles, and thus it quite likely modulated monocytic raft-assembly.
|Institutions:||Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin|
|Keywords:||CD13; Ezetimib; detergent-resistant microdomain|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||08 Feb 2007|
|Last Modified:||20 Jul 2011 20:51|