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An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity

Fischer, Karin and Andreesen, Reinhard and Mackensen, Andreas (2002) An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity. Journal of immunological methods 259 (1-2), pp. 159-69.

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Abstract

The cytotoxic activity of T lymphocytes, natural killer and lymphokine-activated killer cells is usually tested by radioactive assays, which detect the release of cytoplasmic contents after plasma membrane disintegration of dying cells. In contrast to this indirect evaluation of cytotoxicity, we describe here an improved fluorescence assay that is based on the direct quantitative and qualitative ...

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Item Type:Article
Date:2002
Institutions:Medicine > Abteilung für Hämatologie und Internistische Onkologie
Identification Number:
ValueType
11730851PubMed ID
Classification:
NotationType
Annexin A5MESH
Cell Death/immunologyMESH
Cytotoxicity, ImmunologicMESH
Flow Cytometry/methodsMESH
Fluorescent DyesMESH
HumansMESH
Organic ChemicalsMESH
PropidiumMESH
Sensitivity and SpecificityMESH
T-Lymphocytes, Cytotoxic/immunologyMESH
Tumor Cells, CulturedMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:15 Apr 2010 05:13
Last Modified:15 Apr 2010 05:13
Item ID:14358
Owner Only: item control page
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