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Rapid and sensitive detection of CpG-methylation using methyl-binding (MB)-PCR

Gebhard, Claudia and Schwarzfischer, Lucia and Pham, Thu Hang and Andreesen, Reinhard and Mackensen, Andreas and Rehli, Michael (2006) Rapid and sensitive detection of CpG-methylation using methyl-binding (MB)-PCR. Nucleic acids research 34 (11), e82.

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Abstract

Methylation of CpG islands is associated with transcriptional repression and, in cancer, leads to the abnormal silencing of tumor suppressor genes. We have developed a novel technique for detecting CpG-methylated DNA termed methyl-binding (MB)-PCR. This technique utilizes a recombinant protein with high affinity for CpG-methylated DNA that is coated onto the walls of a PCR vessel and selectively ...

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Item Type:Article
Date:2006
Institutions:Medicine > Abteilung für Hämatologie und Internistische Onkologie
Identification Number:
ValueType
10339513PubMed ID
10.1093/nar/gkl437DOI
Classification:
NotationType
Acute DiseaseMESH
Cell Line, TumorMESH
Cells, CulturedMESH
CpG IslandsMESH
DNA MethylationMESH
DNA, Neoplasm/chemistryMESH
DNA-Binding ProteinsMESH
Genes, Tumor SuppressorMESH
HumansMESH
Interferon Regulatory Factors/geneticsMESH
Leukemia, Myeloid/geneticsMESH
Polymerase Chain Reaction/methodsMESH
Promoter Regions, GeneticMESH
Time FactorsMESH
Tumor Cells, CulturedMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:20 Apr 2010 09:38
Last Modified:20 Apr 2010 09:38
Item ID:14480
Owner Only: item control page
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