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Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/7AAD and immunolabeling technique

Endl, E. and Steinbach, P. and Knüchel, R. and Hofstädter, Ferdinand (1997) Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/7AAD and immunolabeling technique. Cytometry 29 (3), pp. 233-241.

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Abstract

Flow cytometric multiparameter analysis of two proliferation associated antigens, Ki-67 and p120, was combined with cell cycle kinetic analysis, achieved by continuous labeling with 5-Bromodeoxyuridine (BrdUrd), followed by staining with Hoechst 33258 and 7-Aminoactinomycin D (7AAD). Exponential and plateau phase monolayer cultures of the human bladder carcinoma cell line J82 were examined. ...

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Item Type:Article
Date:1997
Institutions:Medicine > Lehrstuhl für Pathologie
Identification Number:
ValueType
9389440PubMed ID
Classification:
NotationType
Antigens, Neoplasm/analysisMESH
BisbenzimidazoleMESH
BromodeoxyuridineMESH
Dactinomycin/analogs & derivativesMESH
Flow Cytometry/methodsMESH
Fluorescent Antibody Technique, IndirectMESH
Fluorescent DyesMESH
HumansMESH
Ki-67 Antigen/analysisMESH
Microscopy, FluorescenceMESH
Nuclear Proteins/analysisMESH
Tumor Cells, CulturedMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:17 Jun 2010 08:22
Last Modified:17 Jun 2010 08:22
Item ID:15343
Owner Only: item control page
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