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Stereochemistry and lifetime of the GTP hydrolysis intermediate at the active site of elongation factor Tu from Bacillus stearothermophilus as inferred from the ¹⁷O-⁵⁵Mn superhyperfine interaction

Kalbitzer, Hans Robert and Feuerstein, J. and Goody, R. S. and Wittinghofer, A. (1990) Stereochemistry and lifetime of the GTP hydrolysis intermediate at the active site of elongation factor Tu from Bacillus stearothermophilus as inferred from the ¹⁷O-⁵⁵Mn superhyperfine interaction. European journal of biochemistry: EJB (= theFEBS journal) 188 (2), pp. 355-359.

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Abstract

Electron paramagnetic resonance spectroscopy has been used to obtain information on the structure and stability of the products of GTP cleavage at the active site of elongation factor Tu (EF-Tu) from Bacillus stearothermophilus. Using stereospecifically labelled (Sp)-(Rp)-[beta-17O]GTP (prepared by modification of a previously published procedure which is now also suitable for guanine ...

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Item Type:Article
Date:1990
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
2156700PubMed ID
Classification:
NotationType
Binding Sites/drug effectsMESH
Electron Spin Resonance SpectroscopyMESH
Geobacillus stearothermophilus/analysisMESH
Guanosine Diphosphate/isolation & purificationMESH
Guanosine Triphosphate/isolation & purificationMESH
HydrolysisMESH
Manganese/metabolismMESH
Models, MolecularMESH
Molecular ConformationMESH
Molecular StructureMESH
Oxygen/metabolismMESH
Oxygen IsotopesMESH
Peptide Elongation Factor Tu/isolation & purificationMESH
Phosphates/isolation & purificationMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:03 Sep 2010 12:16
Last Modified:03 Sep 2010 12:16
Item ID:16449
Owner Only: item control page
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