Kalbitzer, Hans Robert and Rohr, G. and Nowak, E. and Goody, R. S. and Kuhn, W. and Zimmermann, H. (1992) A new high sensitivity ¹⁹F probe for labeling cysteine groups of proteins. NMR in biomedicine 5 (6), pp. 347-350.
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A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40 microM solution) can be detected easily in a single scan at 470 MHz.
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer|
|Subjects:||500 Science > 570 Life sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||07 Sep 2010 06:13|
|Last Modified:||07 Sep 2010 06:13|