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A new high sensitivity ¹⁹F probe for labeling cysteine groups of proteins

Kalbitzer, Hans Robert and Rohr, G. and Nowak, E. and Goody, R. S. and Kuhn, W. and Zimmermann, H. (1992) A new high sensitivity ¹⁹F probe for labeling cysteine groups of proteins. NMR in biomedicine 5 (6), pp. 347-350.

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Abstract

A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein ...

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Item Type:Article
Date:1992
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
1489670PubMed ID
Classification:
NotationType
Acetamides/chemical synthesisMESH
Actins/analysisMESH
AnimalsMESH
Cysteine/analysisMESH
Fluorine/analysisMESH
Magnetic Resonance Spectroscopy/methodsMESH
Proteins/analysisMESH
RabbitsMESH
Sensitivity and SpecificityMESH
Spectrum AnalysisMESH
Sulfhydryl Compounds/analysisMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Gertraud Kellers
Deposited On:07 Sep 2010 06:13
Last Modified:07 Sep 2010 06:13
Item ID:16466
Owner Only: item control page
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