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DNA Staining in Agarose Gels with Zn2+ -Cyclen-Pyrene

Schmidt, Florian and Schmidt, J. and Riechers, A. and Haase, S. and Bosserhoff, Anja-Katrin and Heilmann, J. and König, Burkhard (2010) DNA Staining in Agarose Gels with Zn2+ -Cyclen-Pyrene. Nucleosides, Nucleotides & Nucleic Acids (29), pp. 748-759.

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Abstract

A pyrene-labeled Zn²+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amounts as low as 10 ng ...

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Item Type:Article
Date:2010
Institutions:Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König
Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann)
Medicine > Lehrstuhl für Pathologie
Identification Number:
ValueType
10.1080/15257770.2010.515282 DOI
20924956PubMed ID
Keywords:DNA staining; Zn2+-cyclen; pyrene; excimer emission
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Regina Hoheisel
Deposited On:11 Oct 2010 06:40
Last Modified:26 Oct 2010 14:10
Item ID:16993
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