Schmidt, Florian and Schmidt, J. and Riechers, A. and Haase, S. and Bosserhoff, Anja-Katrin and Heilmann, J. and König, Burkhard (2010) DNA Staining in Agarose Gels with Zn2+ -Cyclen-Pyrene. Nucleosides, Nucleotides & Nucleic Acids (29), pp. 748-759.
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A pyrene-labeled Zn²+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amounts as low as 10 ng and short DNA strands (∼300 b.p.) are detectable. Gel extraction as a standard technique in molecular biology was successfully performed after staining with Zn²+-cyclen-pyrene. Cytotoxicity tests on HeLa and V-79 cells reveal that the zinc-cyclen pyrene probe is significant less toxic compared to ethidium bromide.
|Institutions:|| Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König|
Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann)
Medicine > Lehrstuhl für Pathologie
|Keywords:||DNA staining; Zn2+-cyclen; pyrene; excimer emission|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||11 Oct 2010 06:40|
|Last Modified:||26 Oct 2010 14:10|