Haas, E. and Sumper, Manfred
The sexual inducer of Volvox carteri. Its large-scale production and secretion by Saccharomyces cerevisiae.
FEBS letters 294 (3), pp. 282-284.
The DNA sequence coding for the sexual inducer glycoprotein of Volvox carteri and its N-terminal signal peptide was placed under the control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a yeast-E. coli shuttle vector. Yeast transformed by this construct synthesized and secreted into the culture medium biologically active inducer in amounts two to three ...
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