Iuga, Adriana and Spörner, Michael and Ader, Christian and Brunner, Eike and Kalbitzer, Hans-Robert (2006) Rapid assignment of solution ^P NMR spectra of large proteins by solid-state spectroscopy. Biochemical and Biophysical Research Communications 346 (1), pp. 301-305.
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Other URL: http://dx.doi.org/10.1016/j.bbrc.2006.05.116
The application of the 31P NMR spectroscopy to large proteins or protein complexes in solution is hampered by a relatively low intrinsic sensitivity coupled with large line widths. Therefore, the assignment of the phosphorus signals by two-dimensional NMR methods in solution is often extremely time consuming. In contrast, the quality of solid-state NMR spectra is not dependent on the molecular mass and the solubility of the protein. For the complex of Ras with the GTP-analogue GppCH2p we show solid-state 31P NMR methods to be more sensitive by almost one order of magnitude than liquid-state NMR. Thus, solid-state NMR seems to be the method of choice for obtaining the resonance assignment of the phosphorus signals of protein complexes in solution. Experiments on Ras·GDP complexes show that the microcrystalline sample can be substituted by a precipitate of the sample and that unexpectedly the two structural states observed earlier in solution are present in crystals as well.
|Institutions:||Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer|
|Projects:||Graduiertenkolleg Nichtlinearität und Nichtgleichgewicht|
|Keywords:||31P NMR; Phosphate-binding proteins; Solid-state NMR; Liquid-state NMR; Two-dimensional techniques|
|Subjects:||500 Science > 530 Physics|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||20 Mar 2007|
|Last Modified:||20 Jul 2011 21:03|