Babilas, Philipp and Lamby, Philipp and Prantl, Lukas and Schreml, Stephan and Jung, Ernst-Michael and Liebsch, Gregor and Wolfbeis, Otto S. and Landthaler, Michael and Szeimies, Rolf-Markus and Abels, Christoph (2008) Transcutaneous pO2 imaging during tourniquet-induced forearm ischemia using planar optical oxygen sensors. Skin Research And Technology 14 (3), pp. 304-311.
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Background: Oxygen-dependent quenching of luminescence using transparent planar sensor foils was shown to overcome the limitations of the polarographic electrode technique in an animal model. This method was then transferred to a clinical setting to measure the transcutaneous pO2 (ptcO2).
Methods: In six healthy subjects, a cuff on the upper arm was occluded up to 20 mmHg above systolic pressure and released after 8 min. PtcO2 was measured at the lower arm every 30 s before, during, and up to 20 min after cuff occlusion (40 °C applied skin temperature) using luminescence lifetime imaging (LLI) of platinum(II)-octaethyl-porphyrin immobilized in a polystyrene matrix. For validation, the polarographic Clark electrode technique was applied in close proximity, and measurements were conducted simultaneously.
Results: PtcO2 measurements before (70.8±19.1 vs. 66.2±7.7 mmHg) and at the end of ischemic (2.7±1.2 vs. 3.6±1.7 mmHg) and reperfusion phases (72.2±3.6 vs. 68.4±8.9 mmHg) did not differ significantly using the Clark electrode vs. LLI. At both the initial ischemic and the reperfusion phases, the Clark electrode measured a faster decrease or increase, respectively, in ptcO2 because of the oxygen consumption occurring in this method.
Conclusion: The presented method provides accurate and reproducible ptcO2 values under changing microcirculatory conditions. The lack of oxygen consumption during measurement allows both a more realistic estimation of ptcO2 than compared with the gold standard and permanent use in regions with critical oxygen supply.
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||microcirculation; luminescence lifetime imaging; two-dimensional; Clark electrode|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||18 Mar 2011 08:25|
|Last Modified:||21 Jul 2011 04:09|
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