Zhi, Zheng-liang and Drazan, Viktor and Wolfbeis, Otto S. and Mirsky, Vladimir M. (2006) Electrocatalytic activity of DNA on electrodes as an indication of hybridisation. Bioelectrochemistry 68 (1), pp. 1-6.
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Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNA with the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample.
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||DNA; DNA-sensor; Biosensor; Electrocatalysis; Hybridisation; Olygonucleotide; Gene analysis|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||04 Apr 2011 10:30|
|Last Modified:||04 Apr 2011 10:30|